Clontech smarter race manual

The SMARTer RACE 5'/3' Kit has been improved to accommodate larger RNA input volumes and perform better on challenging targets than the original SMARTer RACE cDNA Amplification Kit. The In-Fusion HD Cloning Kit, NucleoSpin Gel and PCR Clean-Up Kit, and Stellar Competent Cells are included for your convenience in cloning RACE products. 10 Rxns. BD Biosciences Clontech www.doorway.ru Protocol No. PT 6 Version No. PR BD SMART™ RACE cDNA Amplification Kit User Manual Figure 2. Overview of the BD SMART™ RACE procedure. Detailed flow charts of the BD SMART RACE mechanisms can be found in Appendices A B. Note that with the cloned RACE fragments. clontech试剂盒 SMARTer_RACE 5'/3' Kit User Manual. Clontech Laboratories, Inc. SMARTer® RACE 5’/3’ Kit User Manual. Cat. No(s). , () Clontech Laboratories, Inc. A Takara Bio Company. Terra Bella Avenue, Mountain View, CA , USA. U.S. Technical Support: United. Asia Pacific Europe Japan Page 1 of 第1页.

SMARTer® RACE 5'/3' Kit User Manual () www.doorway.ru Takara Bio USA, Inc. Page 5 of 30 The SMARTer II A Oligonucleotide contains a terminal stretch of modified bases that anneal to the extended cDNA tail, allowing the oligo to serve as a template for the RT. SMARTScribe RT switches templates from the. User Manual Cat. No. PT (PR5X) Published 7 December Clontech Laboratories, Inc. www.doorway.ru Protocol No. PT Version No. PR5X XI. Rapid Amplification of cDNA Ends (RACE) 28 XII. Characterization of RACE Products 33 XIII. Generation of Full-Length cDNA by PCR 36 XIV. Generation of Full-Length cDNA by. SMARTer™ RACE cDNA Amplification Kit User Manual Cat. Nos. PT () Published January Unite StatesCanaa Asia acific + Additionally, the SMARTer RACE Kit exploits Clontech's patented suppression PCR step-out PCR to increase the sensitivity and reduce the.

Clontech () SMARTer RACE cDNA amplification kit user manual. Clontech, Mountain View, CA, pp 1–33 Google Scholar Kong W, Zhao JJ, He L, Cheng JQ () Strategies for profiling microRNA expression. SMARTer Stranded RNA-Seq Kit User Manual () www.doorway.ru Page 8 of 19 Clontech Laboratories, Inc. A Takara Bio Company. SMARTer Stranded RNA-Seq Kit (96 rxns, Cat. No. ) 96 rxns SMARTer Stranded RNA-Seq Kit (Cat. No. ; Not sold separately) Box 1: µl SMARTer Stranded Oligo (12 μM)*. SMART™ RACE cDNA Amplification Kit User Manual I. Introduction Protocol Overview continued Following reverse transcription, the first-strand cDNA is used directly in 5'- and 3'-RACE PCR reactions, without the need for tedious second-strand synthesis and adaptor ligation. The incorporation of SMART technology also.